Bacterial Genome Sequencing

As of October 2009, the genome sequences of 1,535 bacteria, 49 fungi, and 71 archaebacteria have been published. With NGS technology and a strong bioinformatics platform, BGI provides excellent microbial genome sequencing services. The NGS technology produces more than 100X sequence coverage for most bacterial genomes. The assembly and analysis of a fine map can be completed within 30 working days. The finish time of the perfect map does depend on the number of gaps generated from standard assembly. 

Workflow

Bioinformatics

Data analysis includes:

  1. Genome assembly: statistics of raw data, coverage of the whole genome, and genome assembly
  2. Gene annotation: coding gene prediction, repeat identification, and non-coding RNA prediction
  3. Gene functional annotation: COG classification and KEGG pathway analyses
  4. Synteny analysis and gene family analysis (the genome of the relevant species is required.