Fungal Genome Sequencing

With NGS technology, a fine map of a fungal genome can be completed within two months (with a genome coverage higher than 95%, gene coverage higher than 98%, Scaffold N50>300 kb, and a single base error rate lower than 1/100,000).


Fungal genome sequencing services consists of de novo sequencing and re-sequencing. The method of sequencing analysis and assembly will be performed depending on requirements. For de novo sequencing, we carry out assembly to reach the standards of a fine map as noted above; for re-sequencing, we provide sequencing depths and perform related bioinformatics analysis based on the specific needs indicated by our collaborators. Figure 2-2-2 shows the bioinformatics analysis pipeline used for re-sequencing.



Data analysis includes:

  1. de novo analysis
    1. Genome annotation: Genome composition analysis, gene prediction, repeat identification, non-coding RNA prediction, and pseudogene prediction
    2. Gene functional annotation and classification: Alignment with the sequences using the Blast software
  2. Re-sequencing analysis
    1. Sequencing depth distribution and genome coverage
    2. Consensus sequence assembly
    3. SNP detection and distribution among chromosomes
    4. Structural variation detection and analysis among chromosomes