Sample quantity required (single pair):

1. Short-insert libraries: ≥3 µg
2. 2 kb large-insert libraries: ≥20 µg
3. 5 kb-6 kb large-insert libraries: ≥20 µg
4. 10 kb large-insert libraries: ≥30 µg
5. 20 kb and 40 kb large-insert libraries: ≥60 µg
6. PCR-free libraries with high or low GC content: ≥30 µg

Note: the total sample quantity required is also determined by the experimental strategy, as well as the type and number of libraries to be constructed.

Sample concentration:

1. Short-insert libraries: ≥30 ng/ µL
2. Large-insert libraries: ≥133 ng/ µL

Sample quality: genomic DNA should be intact.
Sample purity: OD260/280= 1.8-2.0

Whole Genome Resequencing:

Plants and Animals Whole Genome Resequencing:

For the genomic DNA samples you will provide:
Purity: OD 260/280=1.8~2.2
Total DNA: Sample quantity demanded: ≥3μg/library
DNA Concentration: ≥50ng/μl, the more the better

Microbial Whole Genome Resequencing:

For the genomic DNA samples you provide us:
Purity: OD260/280=1.8-2.0
Concentration: ≥50 ng/μl
DNA amount: single library preparation starts from at least 5ug, and the total amount should be determined case by case.

Human Whole Genome Resequencing:

Purity: OD260/280=1.8-2.0 without degradation and RNA contamination
Concentration: ≥30ng/μl
DNA amount: For single short insert libraries: ≥6μg. For multiple (library quantity=N) short insert libraries: 3(N+1) μg. The total sample quantity of a single sample with 90 Gb clean data (~30X) for re-sequencing should be no less than 10μg.

Whole Genome Resequencing FFPE Sample Requirements:

Condition: DNA samples without RNA contamination
Purity: OD260/280=1.8~2.0
Input amount: ≥500X(N+1)ng; N represents the number of library construction and 2 libraries are recommended.
For sequencing depth 50X, 5 libraries will be needed, but we recommend 6 libraries.

Bisulfite Sequencing:

For the genomic DNA samples you will provide:
Purity: OD 260/280=1.8-2.0, without DNA degradation and protein pollution
DNA amount: single library preparation starts from at least 5μg

MeDIP Sequencing:

For the genomic DNA samples you will be providing:
Purity:OD260/280=1.8-2.0, without degradation and RNA contamination
Concentration: ≥50ng/μl.
DNA amount: single library preparation starts from at least 10μg, the more the better

ChIP Sequencing:

For the ChIPed DNA samples you will provide:
Purity: OD260/280=1.8-2.0
Concentration: ≥1ng/μl.
Basic Requirements:

1. Gel electrophoresis analysis result should be provided to make sure that the size of DNA fragments is between 100bp to 500bp, and most of the fragments should be about 250bp.
2. DNA total amount: ≥10ng (the more the better) for every single library preparation.

Reduced Representation Bisulfite Sequencing (RRBS):

For the genomic DNA samples you will be providing:
Purity:OD260/280=1.8-2.0, without RNA contamination
Concentration: ≥50ng/μl
DNA amount: single library preparation starts from at least 3μg, the more the better.
RRBS is available for both human and mouse samples

Metagenomic Sequencing:

Metagenomic sequencing should be discussed case by case. A proposal can be provided after we have enough discussion and communication about your project.

Whole Genome Metagenomic Sequencing:

For the microbial genomic DNA you provide us:
Purity:OD260/280=1.8-2.0
Concentration: ≥50ng/μl
DNA amount: single library preparation starts from at least 5μg, and the total amount should be determined case by case.

16S rDNA Tagging

For the PCR products samples you provides us:
Purity: OD260/280:1.8-2.0
Concentration: ≥50ng/μl
DNA amount: single library preparation starts from at least 5μg, and the total amount should be determined case by case.

Exome/Target Sequencing:

Human Exome Sequencing:

For the genomic DNA samples you will provide us:
Purity: OD260/280=1.8~2.0, without degradation and RNA contamination
Sample concentration: ≥30ng/μl
DNA amount: single library preparation starts from at least 6μg, the more the better.

Human Target Region Sequencing:

For the genomic DNA samples you will provide us:
Purity:OD260/280=1.8-2.0, without degradation and RNA contamination
Concentration: ≥30ng/μl
DNA amount: single library preparation starts from ≥30μg (using NimbleGen Sequence Capture Array, targeted region>17Mb) or ≥6μg (using Agilent SureSelect System/NimbleGen Sequence Capture Array when the targeted region is smaller than 17Mb).

Mouse Exome/Target Region Sequencing:

For the genomic DNA samples you will provide us:
Purity: OD260/280=1.8~2.0, without degradation and RNA contamination
Concentration: ≥30ng/μl
Quantity demanded: ≥30μg (using NimbleGen Sequence Capture Array when the targeted region is larger than 17Mb) or ≥6μg (using Agilent SureSelect System or using NimbleGen Sequence Capture Array when the targeted region is smaller than 17Mb).

Monkey Exome Sequencing:

Purity: OD260/280 =1.8~2.0, without degradation and RNA contamination
Concentration: ≥ 50-200 ng/μl
Quantity demanded: ≥ 6 μg (for two times library construction in case there is a failure)

Whole Exome Sequencing FFPE Sample Requirements:

Condition: DNA samples without RNA contamination
Purity: OD260/280: 1.8~2.0
Input amount: ≥2μg
More than 100 folds depth data is recommended.

Genotyping:

For the genomic DNA samples you will provide us:
Purity: OD260/280=1.8-2.0
Concentration: ≥50 ng/μl
DNA amount: single library preparation starts from at least 1μg. DNA quantity demanded for multiple libraries (built N times) 0.5X(N+1) μg.

Optical Mapping:

For the genomic DNA samples you will provide us:
Sample condition: Bacteria DNA with size≥150Kb
Sample concentration: 5-10 DNA/ image
We suggest sending bacterial cultural plate. For bacterial cultural plate, two more control plates are needed for re-culture. Label the culture condition and pathogenicity.
For pathogenic bacteria, we suggest sending Bacteria Plug.

RNA

RNA Sample Requirements

Please provide analysis results of the RNA sample using one or several of the following methods: Qubit, NanoDrop, AGE and Agilent 2100.
Please purify samples, avoiding contamination by polycarbonate, protein and exonuclease. Please clearly state the nature of the solvent used in the sample, in the shipment Sample Information Form.

RNA Sequencing (Transcriptome):

Sample condition: Integrate total RNA samples that have been treated with DNase. Avoid protein contamination during RNA isolation.
Sample quantity (for library construction once):

1. Pant and fungi: total RNA ≥ 20μg
2. Bacteria: total RNA ≥ 5μg
3. Mammal (human, rat and mouse): total RNA ≥ 5μg
4. Other species: total RNA ≥ 10μg

Sample concentration:

1. Plant and fungi: ≥ 250ng/µl
2. Bacteria: ≥ 65ng/µL
3. Mammal (human, rat and mouse): ≥ 65ng/uL
4. Other samples: concentration ≥ 150ng/µl

Sample purity: OD260/280 = 1.8-2.2, OD260/230 ≥ 2.0

1. Plant and fungi: RNA 28S:18S ≥ 1.0, RIN ≥ 6.5
2. Bacteria: RNA 23S:16S ≥ 1.0, RIN ≥ 6.0
3. Animal: RNA 28S:18S ≥ 1.0, RIN ≥ 7.0

RNA Sequencing (Quantification):

Sample condition: Integrated total RNA samples that have been treated with DNase; Avoid protein contamination during RNA isolation
Sample quantity (for library construction): Total RNA ≥ 10 µg
Sample concentration: ≥ 200 ng/µL
Sample purity: OD260/280 = 1.8-2.2; OD260/230 ≥ 1.8; for animal RIN ≥ 7.0, for plant and fungi RIN ≥ 6.5; 28S:18S ≥ 1.0

Quantification FFPE Sample Requirements:

Total RNA: ≥200ng; ≥100ng under the condition of RNA samples after DNAase treatment
RNA concentration: ≥20ng/μl
Purity: OD260/280=1.8~2.2
The FFPE sample can be stored and delivered under room temperature.

small RNA Sequencing:

Sample condition: Integrated total RNA samples. Avoid protein contamination during RNA isolation.

Sample quantity:

1. General requirements: total RNA ≥10 μg
2. For plasma/serum or samples used in Co-IP: total RNA ≥100 ng (≥5mL for serum sample)
3. For < 200 nt small RNA isolated from mirVana™ miRNA Isolation Kit: RNA ≥1μg

Sample concentration:

1. General requirements: concentration ≥200ng/μL
2. For plasma/serum or samples used in Co-IP: concentration ≥ 5ng/μL
3. For < 200 nt small RNA isolated from mirVana™ miRNA Isolation Kit: concentration ≥20 ng/μL

Sample purity: OD260/280 = 1.8-2.2, OD260/230 ≥2.0, for eukaryote except insect 28S:18S ≥1.5, RIN ≥8.0

small RNA FFPE Sample Requirements:

Condition: RNA samples after DNAase treatment
Total RNA: ≥2μg; ≥1μg under the condition of RNA samples after DNAase treatment
RNA concentration: ≥20ng/μl
Purity: OD260/280=1.8~2.2

Proteomics

Proteomics Sample Requirements:

Sample condition: animal tissue, plant tissue, bacteria, cell, body fluid, impurity-rich samples or samples with low content protein and extracts from protein.

Proteome Profiling

Sample quantity demanded (single):
Plant tissue: ≥2g wet weight
Animal tissue: ≥100mg wet weight
Bacteria: ≥200mg wet weight
Fungi: ≥2g wet weight
Body fluid: ≥5 ml
Cell: ≥5×10⁶ cell number
Extracts from protein: ≥500 μg (Concentration ≥1 mg/ml)

Quantitative Proteomics

Sample quantity demanded (single):
Plant tissue: ≥2g wet weight
Animal tissue: ≥100mg wet weight
Bacteria: ≥200mg wet weight
Fungi: ≥2g wet weight
Body fluid: ≥5 ml
Cell: ≥5×10⁶ cell number
Extracts from protein: ≥500 μg (Concentration ≥1 mg/ml)

Phosphoproteomic Analysis

Sample quantity demanded (single):
Plant tissue: ≥20g wet weight
Animal tissue: ≥1g wet weight
Bacteria: ≥2g wet weight
Fungi: ≥20g wet weight
Body fluid: ≥50 ml
Cell: ≥5×10⁷ cell number
Extracts from protein: ≥5 mg (Concentration ≥1 mg/ml)

Protein Identification

SDS-PAGE slices or 2D-PAGE gel particles with a volume ≥ 1.5mm³. Coomassie stain is preferred over silver, but both staining spots should be visible. If using silver, do not fix the gel with glutaraldehyde. Ensure that samples are sent within one week after gel electrophoresis, otherwise, the gel should be kept at 4°C.
Single protein identification:

1. Solution or powder samples which are soluble in water: protein ≥ 10µg.
2. Powder samples that are sparingly soluble in water: protein ≥ 50µg.

Target Proteomics

Sample quantity demanded (single):
Plant tissue: ≥2g wet weight
Animal tissue: ≥100mg wet weight
Bacteria: ≥200mg wet weight
Fungi: ≥2g wet weight
Body fluid: ≥5 ml
Cell: ≥5×10⁶ cell number
Extracts from protein: ≥500 μg (Concentration ≥1 mg/ml)

FFPE

Formalin fixation, paraffin embedding is a conventional storing method for diseased tissue in histopathology. Because formalin-fixed, paraffin-embedded (FFPE) tissues may be stored indefinitely at room temperature and nucleic acids (both DNA and RNA) may be recovered from them decades from the original fixation, FFPE tissues are an important resource for historical studies in medicine.
In the years since our founding, we have greatly expanded our capabilities and now offer you the ability to sequence your FFPE preserved samples. This normally very challenging endeavor enables the study of a wide range of biological and clinical samples and research applications.

To learn more, download the FFPE brochure.

FFPE Sample Requirements (for Human, Mouse and Rat):

 

DNA Level (Whole Exome Sequencing, Target Region Sequencing, Whole Genome Resequencing):

 

WES (Whole Exome Sequencing) and TRS (Target Region Sequencing) FFPE Sample Requirements:

Condition: DNA samples without RNA contamination
Purity: OD260/280: 1.8~2.0
Input amount: ≥2μg
More than 100 folds depth data is recommended.

Whole Genome Resequencing FFPE Sample Requirements:

Condition: DNA samples without RNA contamination
Purity: OD260/280=1.8~2.0
Input amount: ≥500X(N+1)ng; N represents the number of library construction and 2 libraries are recommended.
For sequencing depth 50X, 5 libraries will be needed, but we recommend 6 libraries.

 

RNA Level (Quantification and small RNA):

 

For Quantification:

Total RNA: ≥200ng; ≥100ng under the condition of RNA samples after DNAase treatment
RNA concentration: ≥20ng/μl
Purity: OD260/280=1.8~2.2

For small RNA Sequencing:

Condition: RNA samples after DNAase treatment
Total RNA: ≥2μg; ≥1μg under the condition of RNA samples after DNAase treatment
RNA concentration: ≥20ng/μl
Purity: OD260/280=1.8~2.2

The FFPE sample can be stored and delivered under room temperature.