Methylated DNA immunoprecipitation (MeDIP) is a large-scale (chromosome- or genome-wide) technology that is used for analyzing methylated DNA sequences. Methylated DNA fragments are isolated using an antibody raised against 5-methylcytosine (5mC), the purified fraction of methylated DNA then undergoes next generation sequencing. MeDIP-seq is sensitive to highly methylated, high CpG densities. For the whole genome-wide DNA methylation research, MeDIP is an enrichment-based method, which combined with next-generation sequencing, provides a cost-effective way for DNA methylome studies.
- Comprehensive: Covers all methylated regions across the whole genome
- Cost effective: Enrichment based method, allowing differential analysis of methylated regions across multiple samples with lower cost. Suitable for research of large sample set.
Largest Ever Epigenetics Project Launched
- A collaboration between BGI and TwinsUK
- Capturing the subtle epigenetic signatures that mark the difference between 5,000 twins on a scale and depth never before attempted.
- Exploring how the actions of genes can be temporarily modified by chemical reactions that may occur either at random or by lifestyle or diet
- Looking for differences that explain why many identical twins don't develop the same diseases
- Providing key therapeutic targets for the development of drug treatments.
- Alignment of MeDIP-Seq reads to reference genome
- Distribution for uniquely mapped reads;
- Genome coverage varies with sequencing depth
- Enriched regions – Peaks
- Differentially methylated genes
- Combined Analysis of MeDIP-Seq with small RNA-seq
- Combined analysis of MeDIP-Seq with mRNA expression data
- Combined Analysis of MeDIP-Seq with small RNA-seq and mRNA Expression Data
For the genomic DNA samples you will be providing:
- Purity:OD260/280=1.8-2.0, without degradation and RNA contamination
- Concentration: ≥50ng/μl.
- DNA amount: single library preparation starts from at least 10μg, the more the better.
The standard turnaround time for the workflow, only including standard bioinformatics analysis, is ~45 work days for one sample.