Phosphoproteomic Analysis
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Phosphoproteomic analysis provides identification and quantitation of phosphorylated proteins and peptides, providing key data for understanding their biological function. This technique greatly expands our knowledge of the variety of phosphoproteins, and via rapid analysis techniques, allows us to interrogate the state of entire phosphorylation-based signaling networks. Currently, phosphoproteomic analysis is widely used to research signaling networks, pathological processes, and environmental responses.

Benefits:

  • High accuracy: Capable of localizing a phosphorylation site to a specific amino acid
  • Large-scaleļ¼šThousands of phosphorylation sites can be detected in a single experiment
  • High throughput: Eight samples can be analyzed in a single run by the iTRAQ method
  • Multiple fragmentation methods: Provides CID, HCD, or ETD fragmentation methods
  • Multiple scan model: Provides MS2 or MS3 spectra
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Workflow:

Figure 1. Workflow of Phosphoproteomics Analysis

Bioinformatics:

Standard bioinformatics analysis

  • Database search
  • Statistical analysis of database search results
  • Quality control of database search results
  • Detection and identification of phosphorylated proteins
  • Protein GO, COG and pathway annotation
  • GO and pathway enrichment analysis of phosphorylated proteins

Custom Bioinformatics Analysis

  • We can also perform customized analysis to meet specific project requirements.

Sample Requirements:

Technologies:

  • Mass Spectrometry Platform
    LTQ Orbitrap VelosTM or Triple TOFTM 5600
  • Research Strategy
    TiO2 Enrichment and LC-ESI-MS/MS
  • Standard turnaround time
    ~60 workdays

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