- de novo Sequencing
- Whole Genome Resequencing
- Exome Sequencing
- Target Region Sequencing
- Whole Genome Mapping
- Sanger Sequencing
- Single-Cell DNA Sequencing
- Human MHC-Seq
- Single-Cell Sequencing
- Immune Repertoire Sequencing
- FFPE Samples
de novo sequencing provides the first genome sequence of an organism. With the advent of rapid, low-cost next-generation sequencing technology researchers can now obtain whole genome data for organisms previously considered too low a priority to sequence. The availability of this whole genome data has allowed large-scale genomic studies to be performed that were unimaginable just a few years ago. To date, BGI Tech has sequenced 656 plant and animal reference genomes. The completed projects include rice, cucumber, potato, wheat, silkworm, panda, ant, oyster, minke whale, and so on.
- More comprehensive maps of genetic variation
- Variable gradient insert libraries enable fine mapping of the genome
- Reliable genome assembly by BGI’s independently developed software- SOAPdenovo
- NGS high-throughput sequencing reduces cost
- Experienced bioinformatics team
Comparative Analysis of Bat Genomes Provides Insight into the Evolution of Flight and Immunity. Science. 339: 456-460 (2013).
Comparative analysis of Fruit bat Pteropus alecto and insectivorous Myotis davidii genomes (~ 2 Gb) provides insight into the phylogenetic placement of bats, and moreover reveals evidence of genetic changes that may have contribution to their evolution.
A Heterozygous Moth Genome Provides Insights Into Herbivory and Detoxification. Nature Genetics. 45, 220–225 (2013).
The first genome sequence of the diamondback moth (DBM, 339 Mb) has been published, and this insect is the most destructive pest of brassica crops. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore, and offers insect insights into insect adaptation to host plant and opens new ways for more sustainable pest management.
The Triticum urartu (AA) draft genome sequence (4.94 Gb) provides a diploid reference for analysis of polyploid wheat genomes and is a valuable resource for the evolution, domestication, and genetic improvement of wheat.
Aegilops tauschii Draft Genome Sequence Reveals a gene Repertoire for Wheat Adaptation. Nature. 496, 91–95 (2013).
The Aegilops tauschii draft genome (4.36 Gb) provides novel insights into its role in enabling environmental adaptation of common wheat and in defining the large and complicated genomes of wheat species.
The panda genome was the first genome completely sequenced by next generation sequencing platform alone. It provides clues to the understanding of everything from the panda’s strict bamboo diet to it’s genetic diversity. It may also aid in the panda conservation in the future.
Evolution analysis for animal and plant species
Advanced bioinformatics for microbial species
- Sample quantity required (single pair):
- Short-insert libraries: ≥2.5 µg
- 2 kb large-insert libraries: ≥20 µg
- 5 kb-6 kb large-insert libraries: ≥20 µg
- 10 kb large-insert libraries: ≥30 µg
- 20 kb and 40 kb large-insert libraries: ≥60 µg
- PCR-free libraries with high or low GC content: ≥30 µg
Note: the total sample quantity required is also determined by the experimental strategy, as well as the type and number of libraries to be constructed.
- Sample concentration:
- Short-insert libraries: ≥25 ng/ µL
- Large-insert libraries: ≥133 ng/ µL
- Sample quality: genomic DNA should be intact.
- Sample purity: OD260/280= 1.8-2.0
|Animals/Plants||Survey: 2 months from sample qualification||Common genome: 6 months from sample qualification||Complex genome: 12 months from sample qualification|
|Fungi||Survey: 40 business days||Draft map: 50 business days||Fine map: 50 business days (from completion of survey)|
|Bacteria||Survey: 40 business days||Fine map: 60 business days||Complete map: 75 business days|
Genomic map of plant or animal species (common genome)
|Genome Size (GS)||Assembly Indicator|
|≤ 300 Mb||Contig N50 > 20 kb; Scaffold N50 > 600 kb|
|300 Mb < GS ≤ 1500 Mb (except birds)||Contig N50 > 20 kb; Scaffold N50 > 600 kb|
|1500 Mb < GS ≤ 3000 Mb (except mammals)||Contig N50 > 20 kb; Scaffold N50 > 300 kb|
|Contig N50 > 10 kb; Scaffold N50 > 150 kb|
|GS < 1600 Mb (birds)||Contig N50 > 20 kb; Scaffold N50 > 300 kb|
|GS < 3200 Mb (mammals, except Chiroptera)||Contig N50 > 20 kb; Scaffold N50 > 2 Mb|
Genomic map of microbial species
|Fungi||Survey||Sequencing depth ≥ 30X|
|Draft map||Sequencing depth ≥ 50X|
|Fine map||The coverage of chromosome or chromatin genome is > 95%.The coverage of a gene region is > 98%Scaffold N50 > 300 kb, with an overall sequencing depth ≥ 50X.|
|Bacteria||Survey||Sequencing depth ≥ 100X|
|Fine map||The coverage of chromosome or chromatin genome is > 95%.The coverage of a gene region is above 98%The overall sequencing depth is ≥ 100X.|
|Complete map||Provide 1 contig sequence and PCR validation.|
|Small genome||Survey||Sequencing depth ≥ 100X|