BGI has developed a powerful and highly secure cloud-based genomic computing platform, known as BGI Online, aims to provide easier and more effective solutions for a wide range of problems faced by bioinformaticians. With more robust software, a high level of data security, and well-designed data logistics, BGI Online liberates researchers from the constraints of limited storage space, high labor costs, data delivery, and endless command lines in data analysis.

The advantages of BGI Online:

Powerful, Efficient, and Economical
One-Stop Solution for NGS Research
Collaboration with Anyone, Anywhere
Unparalleled Safety and Security

Powerful, Efficient, and Economical

BGI Online has an integrated infrastructure with high-performance computing servers, massive data storage capabilities, and secure networking interconnection.

• No need to set up and maintain hardware- anytime, anywhere
• Utilize unlimited resources dynamically and Multi-tier, flexible storage
• Seamless integration via automated API
• Powerful metadata system for convenient data/sample management
• Data Level Parallelism (DLP), Automatic monitoring, Smart rerun

One-Stop Solution for NGS Data Analysis

With BGI Online, users can easily combine public bioinformatics tools and our proprietary tools and resources with their own custom tools and data sets to create pipelines that fit their specific needs.

• Ready-to-run Pipelines
  - Analyze your data according to best practices- 5 open genomics pipelines (WGS, WES, RNA-Seq, RNA-ref, single-gene genetic disease); 30+ genomics tools
  - Sophisticated expert support
• Customized Bioinformatics Analysis
  - Full customization for parameters and pipelines
  - Drag-and-drop pipeline construction via an intuitive visual editor
• Downstream Integration
  - Visualization
  - Clinical reporting
• Developer-Friendly
  - Docker-based software development kit (SDK) provides easy and efficient wrapping of tools
  - Supports all major sequencing technologies and all program languages

Collaboration with Anyone, Anywhere

BGI Online users can instantly and securely share data and analysis tools on a global scale, facilitating communication with their collaborators.

• Multiple users can participate in the same project with different access roles: Payer, Uploader, Analyst, and Manager, etc.
• Data and tools can be shared among users
• All activities are recorded and audited
• Leverage pre-collected public data sets and applications
• Publish your data or applications to get rewarded
• Learn from other users via the BBS discussion forum

Unparalleled Safety and Security

BGI Online provides the best-in-class safety performance, ensuring the highest level of data security in the industry and compliance with clinical and diagnostic standards.

• HIPAA ∧EU Data Protection Directive compliant
• Fine-grained project/user privileges
• Secure (SSL) data transfer throughout the platform
• AES256 data encryption at rest

 

Get Started Today

Go to www.bgionline.com to sign up for your free BGI Online account. Every new user gets 30,000 free credits to explore the BGI Online cloud analysis platform.

Human cancers typically carry several genomic rearrangements, such as copy number alterations and point mutations. These genetic alterations are important diagnostic, prognostic, and predictive biomarkers to help design personalized treatments thatare more effective and less toxic.

BGI TumorCareTM is a newly introduced NGS-based panel solution designed to detect all types of tumor genomic alterations, including base substitutions, insertions and deletions, copy number alterations, and selected fusions. BGI, leveraging our years of cancer research experience combined with extensive literature and database searches, has designed a comprehensive cancer gene panel. BGI TumorCareTM includes 1053 genes that (1) are strongly supported as clinically-actionable cancer genes, (2) harbor high frequency alterations in the COSMIC database, or (3) play key roles in core cancer pathways.

Followed by target region sequencing and our bioinformatics analysis pipeline, all types of alterations are detected with high sensitivity and specificity (Table 1). Moreover, using our extensive in-house cancer knowledgebase, including Drug-Bank database, PharmaGKB database, and cancer research literature, this panel allows researchers to identify and investigate the associations between detected genomic alterations and 117 known cancer therapeutics, including those approved by FDA and in clinical trials. This knowledge may help us understand more about the effects and cytotoxicity of specific treatments, such as target therapy and chemotherapy.

Benefits:

• Comprehensive detection: SNVs, INDELS, CNVs, and gene fusions of 1053 genes in a single assay for a wide variety of cancer types
• High sensitivity and specificity: >99% in ≥1% minor allele frequencies (Table 1)
• Low input DNA amounts as low as 200 ng
• Enables in-depth investigation of genetic variations and their associations to target therapies and traditional chemotherapies through our extensive knowledgebase.
• Experienced team: Our team has analyzed tens of thousands of cancer samples and has many publications in top-tier journals
• Affordable cost per sample: Contact us to learn more.
• FFPE, normal tissue, biopsy, and single-cell samples supported

Table 1. Accurate and sensitive detection of mutations

 

Note: Data are from standard tumor samples with known alterations (108 SNPs and 60 INDELS) without matched normal tissues. Sequencing was performed using HiSeq200 pair-end 101 with an initial DNA amount of 200 ng.

For research use only. Not for therapeutic or diagnostics use.

Equipped with cutting-edge technologies, BGI Tech brings extensive know-how and experience to the field of Autoimmune Diseases Research. Our MHC Capture Sequencing (co-developed by BGI and Roche NimbleGen) and Immune Repertoire Sequencing services provide you with solutions for biomarker discovery, disease progression analysis, pathogenesis research and drug development.

Best of Our Immune Research Products
Human MHC Capture Sequencing technology is a top-level HLA typing capture kit. This new MHC region capture technology not only targets traditional MHC region (3.37MB), but also covers approximately 1.6Mb of the regions surrounding MHC, providing a total of 4.97Mb (chr6: 28477797-33448354) and including 8 known haplotypes. This method will help advance research and development of new medicines for immune diseases.	Immune Repertoire Sequencing (IR-SEQ) is to amplify the complimentary determining region (CDR) of B-cell receptor (BCR) or T-cell receptor (TCR) using multiple-PCR or 5′ RACE methods, followed by high-throughput sequencing. It is used to comprehensively assess the diversity of the immune system by identifying lymphocyte receptor gene structures and profiling the expression of lymphocyte clones, and to study the underlying mechanisms and functions.
Benefits: High coverage: >97% of MHC and extended regions Accurate variation detection: >99% consistency with SNP genotyping results Accurate HLA typing: >95% of 26 HLA genes can be typed at 4-digit resolution Reference: An Integrated Tool to Study MHC Region: Accurate SNV Detection and HLA Genes Typing in Human MHC Region Using Targeted High-Throughput Sequencing. Cao H et al. PLoS One. DOI: 10.1371/journal.pone.0069388 (2013).	Benefits: One-stop service: Amplification of CDR/CDR3, library construction, sequencing, bioinformatics analysis and data statistics. Specific methods: Specific primers for CDR3 V region and C region to amplify the CDR3 region, among which TRA, IGH, IGK/IGL have been applied for patents successfully. High-fidelity amplification methods: Multiple-PCR or 5′ RACE methods to amplify different clones; the number of unique clones is as large as 105.
Promotion Offer The immune repertoire is mainly affected by the following three aspects: 1) Polymorphism of MHC loci; 2) Contact of antigens; 3) Consistent regulation of the immune system. BGI provides a new solution, which combines immune repertoire sequencing and MHC capture sequencing, to find the relationship between individual or population immune repertoire and HLA types. We offer validation of your immune repertoire sequencing results by MHC capture sequencing and you can get 50% off for MHC when you do immune repertoire sequencing with us. If you require more comprehensive analysis, we offer Human AllinOne Capture Sequencing to detect not only HLA genes but all exomes. In addition, Our AllinOne Sequencing also captures Han tagSNPs for Chinese population.  More information: Human MHC: http://bgiamericas.com/service-solutions/genomics/human-mhc-seq/ Human AllinOne : http://bgiamericas.com/service-solutions/genomics/allinone/ Immune Repertoire Sequencing: http://bgiamericas.com/service-solutions/immune-repertoire-sequencing/

New features of RNA-Seq transcriptome data analysis are introduced to provide you with more insightful information regarding differentially-expressed genes (DEGs), exons, RNA editing, and protein interactions.

Personalized service

We can also perform customized analyses to meet your specific research needs.

Contact us to learn more!

Introduction
BGI Tech has launched the Agilent SureSelectXT Human Methyl-Seq service, a cost-effective approach for analyzing the methylation status of cytosine sites of specific regions within the human genome.

This approach combines Agilent SureSelect target region enrichment platform with bisulfite sequencing- the gold standard for DNA methylation analysis, and enables the most comprehensive coverage of disease related regions to accelerate your epigenetic studies and complex disease research.

Designed contents:
84Mb design, 3.7M CpGs
CpG islands
known cancer tissue specific DMRs
GENCODE promoters
CpG islands shelves (~4kb away from islands)
DNase I hypersensitive sites
Refseq genes
Ensembl regulatory features

Advantages:
Cost-effective to explore the methylation status of specific regions
Single base resolution
Excellent concordance with WGBS data
High reproducibility
Thorough investigation of known and novel DMRs

Applications:
Epigenome-wide association studies (EWAS)
Cancer research
Cell development study
Signaling pathway analysis

Contact us to learn more!

Nowadays sequencing of specific genomic region has been successfully applied to the investigation of genetic diseases and large-scale validation of candidate genes responsible for complex diseases. To facilitate your research, BGI provides the WaferGen’ target enrichment system-PCR-based amplification, in addition to probe hybridization used in targeted sequencing.

Benefits Low input Only 100pg gDNA per amplicon Less amplification bias Reduce amplification bias by Singleplex PCR and ensure that each target sequence is amplified in a separate PCR reaction Fast turnaround time Amplify 5184 amplicons within 4 hours by machinary loading PCR reagents to sequencer-ready amplicon, requiring only minimal user intervention Cost effective As low as 0.50 USD per amplicon

Workflow

DNA Sample RequirementsAmount: ≥0.5μg; Concentration: ≥37.5ng/uL; Purity: OD260/280= 1.8~2.0.

Turnaround TimeThe standard turnaround time can be as fast as 6~8 weeks for up to 1000 samples including chip loading, sequencing, and bioinformatics analysis.

Bioinformatics AnalysisA Bioinformatics analysis is the same as our “Target Region Sequencing” service.

BGI Tech’s Whole Transcriptome RNA-Seq service enables investigation of both mRNAs and long non-coding RNAs on the same samples. With our ongoing effort to improve our services, you can now experience the following upgraded services at no extra cost. Contact us to learn more!

Examples of newly added analyses:

1. Differential gene expression analysis across all samples

2. Differential gene expression analysis between groups of samples

3. Gene expression pattern analysis

4. lncRNA function prediction
     4.1 lncRNA-mRNA interaction analysis

    
4.2 Investigation of up- or down-stream lncRNA of a gene

 

    
4.3 Pre-miRNA prediction

Contact us to learn more!

Exome sequencing, an innovative exome-targeted research technology, is the fastest and one of the most useful technologies for searching for variants that may cause diseases. In order to continue providing superior service, BGI has upgraded its whole exome sequencing product and now delivers additional bioinformatics analysis services at no extra cost. Of particular note, we have updated our advanced and customized analysis to provide more comprehensive and superior analyses suitable for research in cancer, complex disease, population genetics, and Mendelian disorders.

Cancer

1、We now provide preliminary identification for paired tumor-normal samples based on MassARRAY, to avoid incorrect information of pair samples.
2、CancerGeneCensus database is added for non-synonymous annotation of mutated genes.

Complex Diseases

1、A rigorous and robust bioinformatics analysis pipeline is developed to perform strict quality control for large-scale exome sequencing data sets. The pipeline includes population- level SNP calling, LD-based genotyping calling, systematic sample QC and SNP site QC, and SNP association.
2、OMIM database has been added for SNP annotation.

Complex Diseases (Customized Solutions)

1、A case-control matching method is now used to control population stratification to ensure genetic homogeneity of the sample.
2、Pathway-based protein interaction analysis (pairwise or multiple interaction) is now included to detect interacting genes.
3、eQTL analysis has been added to find more reliable causal SNPs.

Population Genetics

1、Population SNP QC and sample QC is performed to find more reliable SNPs.
2、Multiple selection detection methods, including iHS, XP-EHH, and DDAF, are used to ensure reliable results.
3、Pathway analysis and haplotype analysis have also been added, facilitating the mining of information at a deeper level.

Want to know more about our Exome Sequencing Services? Please click here.

BGI Tech is proud to introduce NGS-based Molecular Breeding, a technology that leverages powerful properties of NGS and molecular breeding in order to accelerate breeding programs and scientific research.

Using this new platform, you can complete your breeding work within one year as opposed to the six to eight years required with traditional breeding methods. You will dramatically save the time and cost. BGI Tech has successfully assisted customers in completing several breeding projects in the species of Rice, Melon and one new Foxtail Millet improved hybrid.

Advantages:

• High-density markers and high-accuracy genetic maps.
• Cost-effective for establishing the new platform.
• Time-effective where breeding could be completed in less than a year.
• Scientific advice for species specific breeding.
• Database construction and cloud computing.
• More diversified and comprehensive study protocols for customers.

 

About BGI Tech