The promotion is valid for Americas and Europe customers only NOW through JULY 31!
Whole Genome Bisulfite Sequencing (WGBS) - 40% OFF
Bisulfite sequencing is the gold standard for DNA methylation analysis. Coupled with next-generation sequencing technology, it allows for an unbiased, genome-wide analysis of DNA methylation. In human health and disease research, the DNA methylation status of all cytosines is paramount for understanding the mechanisms of many biological processes. In animal and plant research, it can be adapted to study the influence of nutrition, environment, and natural selection on DNA methylation, providing a solid foundation for molecular breeding.
Advantages:
- Accurate analysis of methylation patterns of every cytosine
- Generates a complete methylome map for any species
- Recognized as the gold standard for DNA methylation analysis
The promotion includes the following services:
- Library construction, sequencing, and standard bioinformatics analysis.
- For human, the price is 7,740 USD/per sample for 60 Gb of clean data.
- For animal and plant, the price of each sample depends on the desired amount of sequencing data.
- 91bp paired-end sequencing on HiSeq 2000.
Standard bioinformatics analysis includes:
- Data Filtering and production statistics
- Reads alignment
- Sequence depth and coverage analysis
- Calculation of Methylation Level
- Global trends of methylome
- Genome-wide methylation profiling
- Identification of differentially methylated regions (DMRs)
Turnaround time: 50 business day for up to 4 samples
Our Experience:
To data, BGI has successfully completed WGBS for multiple species, including human, silkworm, rat, mouse, arabidopsis, zebra fish, maize, rice, bee, chicken, etc.
BGI Publications:
[1] Li Y, Zhu J, Tian G, Li N, et al. The DNA methylome of human peripheral blood mononuclear cells. PLoS Biol. 2010 8(11).
[2] Xiang H, Zhu J, Chen Q, et al. Single base-resolution methylome of the silkworm reveals a sparse epigenomic map. Nat Biotechnol. 2010, 28(5):516-20.
[3] Heyn H, Li N, Ferreira HJ, et al. Distinct DNA methylomes of newborns and centenarians.Proc Natl Acad Sci2012, 109(26):10522-7.