- de novo Sequencing
- Whole Genome Resequencing
- Exome Sequencing
- Target Region Sequencing
- Genotyping by Sequencing
- Whole Genome Mapping
- Sanger Sequencing
- Single-Cell DNA Sequencing
- Human MHC-Seq
- Single-Cell Sequencing
- Immune Repertoire Sequencing
- FFPE Samples
Fosmid Library Construction
Construction of a fosmid library uses an improved cosmid plasmid vector. The vector system is constructed after introducing pBAC to PUCcos and fusing.
Features of the vector system:
- Due to the insertion of the E. coli fertility factor (F-factor), it exists in the host bacteria as a single copy form and is very stable;
- The vector has an induced oriV high-copy, replication starting point. If needed, it can be induced to high copy (about 50);
- The high randomness ensures equal frequency of every fragment of the DNA in the library;
- The inserted fragments have high uniformity, generally about 40Kb.
- Construction of whole-genome physical maps;
- Research of gene function and gene expression regulation;
- Map-based cloning of genes conferring important traits;
- Gene structure and function analysis.
The main results of library qualification include storage capacity, insert segment size, homologous alignment, and randomness analysis.
- Sample condition: Samples should be of DNA or fresh tissue. Plant samples should be etiolated seedlings.
- Sample concentration and purity: The concentration should be ≥ 50 ng/μL, the main band of genomic DNA ≥ 40 kb, the total DNA ≥ 50 μg, and OD260/280 = 1.8 to 2.0. The DNA solutions should be free of visible contamination.
The standard turnaround time for library construction using the workflow (above) is 30 working days.
Completion Milestone and Results Submission:
The project is considered complete when the insert fragment size is approximately 40 kb, the empty clones rate is ≤ 5%, and the capacity of the library=genome size × coverage/insert fragment size.
Results include 96 or 384 bacterial preservation plates and a library inspection report.