- de novo Sequencing
- Whole Genome Resequencing
- Exome Sequencing
- Target Region Sequencing
- Genotyping by Sequencing
- Whole Genome Mapping
- Sanger Sequencing
- Single-Cell DNA Sequencing
- Human MHC-Seq
- Single-Cell Sequencing
- Immune Repertoire Sequencing
- FFPE Samples
Target sequences are amplified in different individuals with PCR. Then the amplified products are sequenced and analyzed. PCR re-sequencing has the following applications:
- Single-gene genetic diseases: pathogenic gene research and gene diagnosis;
- Complex diseases: relevant gene research and drug susceptibility analysis;
- Association analysis between the phenotypes and nucleic acid level variation with mutation, insert, delete, etc.
Bioinformatics contents consist of basic analysis based on the PloyPred software.
- DNA templates and primers required for PCR or sequencing:
Requirements are a genome DNA concentration of 0.1-1 μg/µL, with a volume of ≥ 20 μL per PCR reaction. The primers should be dry powder (1 OD).
- PCR products:
Sample concentration should be ≥ 50 μg/µL for unpurified PCR product and ≥ 20 μL; for purified PCR product. The corresponding gel electrophoresis result should be provided.
The turnaround time is determined by the number of samples and sequences.
Completion Milestone and Result Submission:
Project completion is based on a quality score of Q 20.
Results consist of a sequencing peak figure, basic analysis, and the final report.