Complete Bacterial Genome Map
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The complete map of a bacterial genome provides researchers with the complete genome sequence. Based on a complete bacterial genome map, researchers can conduct detailed investigations of key genes and proteins to reveal their specific functions and predict molecular mechanisms. In addition, researchers can use a complete genome map to investigate the bacterial pathogenicity and patterns of interaction with the host, which facilitates the development of vaccines and novel antibiotics and aids the elucidation of bacterial evolutionary patterns.

Platform Options

In addition to the Illumina platform, we also provide services based on the BioNano and PacBio platforms.

De novo assembly of BioNano genome maps yields high-fidelity contiguous map information across long ranges. This can greatly improve the assembly of sequences that are built from relatively short second- and third-generation reads, particularly in highly repetitive regions of the genome.

The PacBio RS single-molecule, real-time sequencing system provides extra-long read lengths that simplify and improve genome assembly irrespective of GC content.

Genome Sequencing of 161 Mycobacterium tuberculosis Isolates From China Identifies Genes and Intergenic Regions Associated With Drug Resistance. Nature Genetics. 45, 1255–1260 (2013).

The worldwide emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis threatens to make this disease incurable. Here we sequenced and analyzed 161 isolates with a range of drug resistance profiles, discovering 72 new genes, 28 intergenic regions (IGRs), 11 nonsynonymous SNPs and 10 IGR SNPs with strong, consistent associations with drug resistance. Our work indicates that the genetic basis of drug resistance is more complex than previously anticipated and provides a strong foundation for elucidating unknown drug resistance mechanisms.

Bioinformatics:

  1. Genome assembly
  2. Genome component analysis (gene components, repeat sequences, non-coding RNAs, clustered regularly interspaced short palindromic repeats (CRISPR), prophages, and genomic islands)
  3. Gene function analysis
    1. General gene annotation (default databases: KEGG, Swissprot, and GO; optional databases: Nr and COG)
    2. Analyses of the pathogenicity and drug resistance of animal pathogens
    3. Analyses of the pathogenicity of plant pathogens
  4. Comparative genomic analysis
    1. Genomic homologies and consensus sequences
    2. SNPs and InDels
    3. Structural variations
    4. Core or pan genomes
    5. Evolution analysis

Sample Requirements:

  1. Sample quantity (for one round of library construction):
    1. Short-insert libraries: ≥2.5 µg
    2. Large-insert libraries: ≥10 µg
  2. Sample concentration: ≥30 ng/µL
  3. Sample purity: OD260/280 = 1.8~2.0; OD260/230 = 2.0~2.2

Turnaround Time:

The standard turnaround time for the workflow (above) is 30-50 business days.

Completion Criteria:

Provide 1 contig with no gaps