- de novo Sequencing
- Whole Genome Resequencing
- Exome Sequencing
- Target Region Sequencing
- Genotyping by Sequencing
- Whole Genome Mapping
- Sanger Sequencing
- Single-Cell DNA Sequencing
- Human MHC-Seq
- Single-Cell Sequencing
- Immune Repertoire Sequencing
- FFPE Samples
- Best assembly quality with the highest N50 scaffold and contig sizes provided
- More comprehensive maps of genetic variation
- Variable gradient insert libraries that enable fine mapping of the genome
Comparative Analysis of Bat Genomes Provides Insight into the Evolution of Flight and Immunity. Science. 339: 456-460 (2013).Comparative analysis of Fruit bat Pteropus alecto and insectivorous Myotis davidii genomes (~ 2 Gb) provides insight into the phylogenetic placement of bats, and moreover reveals evidence of genetic changes that may have contribution to their evolution.
A Heterozygous Moth Genome Provides Insights Into Herbivory and Detoxification. Nature Genetics. 45, 220–225 (2013).The first genome sequence of the diamondback moth (DBM, 339 Mb) has been published, and this insect is the most destructive pest of brassica crops. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore, and offers insect insights into insect adaptation to host plant and opens new ways for more sustainable pest management.
Aegilops tauschii Draft Genome Sequence Reveals a gene Repertoire for Wheat Adaptation. Nature. 496, 91–95 (2013).The Aegilops tauschii draft genome (4.36 Gb) provides novel insights into its role in enabling environmental adaptation of common wheat and in defining the large and complicated genomes of wheat species.
To provide better quality service for our customers, we offer two types of plant and animal de novo sequencing services, X bio and Intelligen.
X bio, which includes genome survey, sequencing, and genome assembly, is best for those customers with some bioinformatics knowledge and the ability to conduct further analysis of the assembly results themselves.
Intelligen is suitable for those customers with greater needs for bioinformatics analysis, and we can provide end-to-end solutions for your specific research needs. This service includes what is offered in X bio plus gene annotation, evolution analysis, and any other personalized analysis.
- Library construction strategies:
- Paired-end library construction strategy with insert size of 170 bp, 500 bp, and 800 bp
- Long-span mate-pair library construction strategy with insert size of 2 kb, 5 kb, 10 kb, 20 kb, and 40 kb
- Sequencing strategy：
- The average sequence depth is greater than 80X.
- Sequencing strategy of pair end library: 91PE or 101PE sequencing
- Sequencing strategy of mate-pair library: 50PE or 91PE sequencing
- Sequencing using PacBio’s single molecule real-time (SMRT) technology is also available for specific research needs.
X bio -- Best for those customers with some bioinformatics knowledge
Intelligen -- Best for those customers with greater needs for bioinformatics analysis
- Sample quantity required (single pair):
- Short-insert libraries: ≥3 µg
- 2 kb large-insert libraries: ≥20 µg
- 5 kb-6 kb large-insert libraries: ≥20 µg
- 10 kb large-insert libraries: ≥30 µg
- 20 kb and 40 kb large-insert libraries: ≥60 µg
- Sample concentration:
- Short-insert libraries: ≥30 ng/ µL
- Large-insert libraries: ≥133 ng/ µL
- Sample purity: OD260/280= 1.8-2.0
|Services||X bio||Intelligen – Common genome||Intelligen – Complex genome|
|Turnaround time||4–9 months (depending on genome complexity)||6 months||1 year|
|Birds , Mammals (except Chiroptera)||Contig N50 ≥ 30 kb; Scaffold N50 ≥ 2 Mb|
|Plants||Contig N50 ≥ 30 kb; Scaffold N50 ≥ 800 kb|
|Complex genome||Contig N50 ≥ 20 kb; Scaffold N50 ≥ 300 kb|
|Others||Contig N50 ≥ 20 kb; Scaffold N50 ≥ 600 kb|